Epigenetic Changes of Serotonin Transporter in the Patients with Alcohol Dependence: Methylation of an Serotonin Transporter Promoter CpG Island
Psychiatry Investigation
;
: 130-133, 2011.
Article
in English
| WPRIM
| ID: wpr-35977
ABSTRACT
OBJECTIVE:
Psychiatric disorders such as depression, anxiety and alcohol dependence are associated with serotonin metabolism. We assessed the methylation level of the serotonin transporter (5-HTT) promoter region in control and alcohol dependent patients.METHODS:
Twenty seven male patients who met the Diagnostic and Statistical Manual of Mental Disorder IV (DSM-IV) criteria for alcohol dependence were compared with fifteen controls. Polymerase chain reaction (PCR) assays of bisulfate-modified DNA were designed to amplify a part of the CpG island in the 5HTT gene. Pyrosequencing was performed and the methylation level at seven CpG island sites was measured.RESULTS:
We found no differences in the methylation patterns of the serotonin transporter linked promoter region (5-HTTLPR) between alcohol-dependent and control subjects.CONCLUSION:
Our negative finding may be because 5-HTT epigenetic variation may not affect the expression for 5-HTT or there may be other methylation site critical for its expression. To find out more conclusive result, repeating the study in more methylation sites with a larger number of samples in a well-controlled setting is needed.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Anxiety
/
DNA
/
Serotonin
/
Polymerase Chain Reaction
/
Promoter Regions, Genetic
/
CpG Islands
/
Depression
/
Alcoholism
/
Serotonin Plasma Membrane Transport Proteins
/
Epigenomics
Type of study:
Diagnostic study
/
Practice guideline
Limits:
Humans
/
Male
Language:
English
Journal:
Psychiatry Investigation
Year:
2011
Type:
Article
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