Molecular mechanism of epididymal protease inhibitor modulating the liquefaction of human semen / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
;
(6): 770-775, 2008.
Article
in English
| WPRIM
| ID: wpr-359911
ABSTRACT
<p><b>AIM</b>To study the molecular mechanism of epididymal protease inhibitor (Eppin) modulating the process of prostate specific antigen (PSA) digesting semenogelin (Sg).</p><p><b>METHODS</b>Human Sg cDNA (nucleotides 82-849) and Eppin cDNA (nucleotides 70-723) were generated by polymerase chain reaction (PCR) and cloned into pET-100D/TOPO. Recombinant Eppin and Sg (rEppin and rSg) were produced by BL21 (DE3). The association of Eppin with Sg was studied by far-western immunoblot and radioautography. In vitro the digestion of rSg by PSA in the presence or absence of rEppin was studied. The effect of anti-Q20E (N-terminal) and C-terminal of Eppin on Eppin-Sg binding was monitored.</p><p><b>RESULTS</b>Eppin binds Sg on the surface of human spermatozoa with the C-terminal of Eppin (amino acids 75-133). rSg was digested with PSA and many low molecular weight fragments were produced. When rEppin is bound to rSg, then digested by PSA, incomplete digestion and a 15-kDa fragment results. Antibody binding to the N-terminal of rEppin did not affect rSg digestion. Addition of antibodies to the C-terminal of rEppin inhibited the modulating effect of rEppin.</p><p><b>CONCLUSION</b>Eppin protects a 15-kDa fragment of rSg from hydrolysis by PSA.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Semen
/
Spermatozoa
/
Autoradiography
/
Recombinant Proteins
/
Prostate-Specific Antigen
/
Cell Biology
/
Seminal Vesicle Secretory Proteins
/
Allergy and Immunology
/
Proteinase Inhibitory Proteins, Secretory
Limits:
Animals
/
Humans
/
Male
Language:
English
Journal:
Asian Journal of Andrology
Year:
2008
Type:
Article
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