Effects of plant extract neferine on cyclic adenosine monophosphate and cyclic guanosine monophosphate levels in rabbit corpus cavernosum in vitro / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
;
(6): 307-312, 2008.
Article
in English
| WPRIM
| ID: wpr-359971
ABSTRACT
<p><b>AIM</b>To further investigate the relaxation mechanism of neferine (Nef), a bis-benzylisoquinoline alkaloid extracted (isolated) from the green seed embryo of Nelumbo nucifera Gaertn in China, on rabbit corpus cavernosum tissue in vitro.</p><p><b>METHODS</b>The effects of Nef on the concentrations of cyclic adenosine monophosphate (cAMP) and cyclic guanosine monophosphate (cGMP) in isolated and incubated rabbit corpus cavernosum tissue were recorded using 125I radioimmunoassay.</p><p><b>RESULTS</b>The basal concentration of cAMP in corpus cavernosum tissue was 5.67 +/- 0.97 pmol/mg. Nef increased the cAMP concentration in a dose-dependent manner (P < 0.05), but this effect was not inhibited by an adenylate cyclase inhibitor (cis-N-[2-phenylcyclopentyl]azacyclotridec-1-en-2-amine, MDL-12, 330A) (P > 0.05). The accumulation of cAMP induced by prostaglandin E1 (PGE1, a stimulator of cAMP production) was also augmented by Nef in a dose-dependent manner (P < 0.05). The basal concentration of cGMP in corpus cavernosum tissue is 0.44 +/- 0.09 pmol/mg. Nef did not affect this concentration of cGMP, either in the presence or in the absence of a guanyl cyclase inhibitor (1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one, ODQ) (P > 0.05). Also, sodium nitroprusside (SNP, a stimulator of cGMP production)-induced cGMP production was not enhanced by Nef (P > 0.05).</p><p><b>CONCLUSION</b>Nef, with its relaxation mechanism, can enhance the concentration of cAMP in rabbit corpus cavernosum tissue, probably by inhibiting phosphodiesterase activity.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Penis
/
Pharmacology
/
Seeds
/
Vasodilator Agents
/
In Vitro Techniques
/
Penile Erection
/
Radioimmunoassay
/
Drugs, Chinese Herbal
/
Plant Extracts
/
Cyclic AMP
Limits:
Animals
Language:
English
Journal:
Asian Journal of Andrology
Year:
2008
Type:
Article
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