Disruption of ectoplasmic specializations between Sertoli cells and maturing spermatids by anti-nectin-2 and anti-nectin-3 antibodies / 亚洲男科学杂志(英文版)
Asian Journal of Andrology
;
(6): 577-584, 2008.
Article
in English
| WPRIM
| ID: wpr-359979
ABSTRACT
<p><b>AIM</b>To understand the biological functions of the ectoplasmic specializations between Sertoli cells and maturing spermatids in seminiferous epithelia.</p><p><b>METHODS</b>In order to disrupt the function of the ectoplasmic specializations, nectin-2, which is expressed at the specialization, was neutralized with anti-nectin-2 antibody micro-injected into the lumen of the mouse seminiferous tubule. Anti-nectin-3 antibody was also micro-injected into the lumen in order to neutralize nectin-3, which is expressed at the specialization.</p><p><b>RESULTS</b>The actin filaments at the specialization disappeared, and exfoliation of maturing spermatids was observed by electron microscopy.</p><p><b>CONCLUSION</b>Nectin-2 was neutralized by anti-nectin-2 antibody and nectin-3 was neutralized by anti-nectin-3 antibody, respectively. Inactivated nectin-2 and nectin-3 disrupted the nectin-afadin-actin system, and finally the actin filaments disappeared. As a result, the specialization lost the holding function and detachment of spermatids was observed. One of the functions of the specialization seems to be to hold maturing spermatids until spermiation.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Seminiferous Epithelium
/
Sertoli Cells
/
Spermatids
/
Cell Adhesion Molecules
/
Cell Communication
/
Actins
/
Microscopy, Confocal
/
Cell Biology
Limits:
Animals
Language:
English
Journal:
Asian Journal of Andrology
Year:
2008
Type:
Article
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