Factor Xa Promotes Differentiation of Meg-01 Cell Line / 中国实验血液学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effect and mechanism of Factor Xa on the differentiation of Meg-01 cells into platelet-like particles.</p><p><b>METHODS</b>The Meg-01 cells were used as experimental object, Factor Xa was used as agonist. Cell proliferation was detected by CCK-8 assay. The viability of platelet-like particles was analyzed by AlamaBlue kit. MAPK/ERK pathway and PI3K/AKT pathway were assayed by Western blot. The expression of CD41b was analyzed by Western blot and flow cytometry. Cell cycle and apoptosis were detected by flow cytometry.</p><p><b>RESULTS</b>The Factor Xa (1 µg/ml) inhibited cell viability, induced apoptosis. Factor Xa triggered cell arrest at the G(2)/M stage and down-regulated the expression of SKP2. After Meg-01 cells were stimulated by Factor Xa, the expression of CD41b was up-regulated and the MAPK/ERK pathway and PI3K/AKT pathway were activated. The platelets-like particles stimulated by FXa activation were viable.</p><p><b>CONCLUSION</b>The Factor Xa maybe display some effect on the differentiation of megakaryocytes into platelets.</p>