Effect of Magnolol on Proliferation and Apoptosis of HL-60 Cells and Its Molecular Mechanism / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 388-393, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-360080
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of magnolol on proliferation and apoptosis of HL-60 cells and its mechanism.</p><p><b>METHODS</b>MTT assay was used to measure the proliferation of HL-60 cells after treatment with different concentration of magnolol (5, 10, 20, 40, 80 and 160 µg/ml). The morphological changes of HL-60 cells were examined by light microscopy, and DAPI staining was performed to observe the nuclear morphology of HL-60 cells. The early cell apoptosis was detected by flow cytometry with Annexin V-FITC/PI double-staining. RT-PCR was carried out to examine the mRNA expression of BAX and BCL-2. Western blot was performed to detect the protein expression of caspase family.</p><p><b>RESULTS</b>The magnolol inhibited HL-60 cell proliferation, and the inhibitory rate of cell proliferation increased significantly in a dose- and time- dependent manner (P < 0.05). HL-60 cells became small, even apoptotic bodies appeared after treatment with magnolol. In addition, nuclear condensation or fragmentation could be observed, which is the typical morphological features of apoptosis. When HL-60 cells were treated with 40 µg/ml of magnolol for 24 h, the ratio of early apoptotic cells reached to (11.7 ± 2.4) %, which was significant different from control (1.4 ± 1.1) % (P < 0.05). RT-PCR results showed that treatment of HL-60 cells with magnolol up-regulated the expression of BAX, whereas down-regulated the expression of BCL-2. Western blot results showed that the cleavages of caspase-3, -8 and -9 were significantly enhanced by magnolol.</p><p><b>CONCLUSION</b>The magnolol can significantly inhibit the proliferation of HL-60 cells and induce the apoptosis of HL-60 cells, which may occur through up-regulation of BAX, down-regulation of BCL-2 and the activation of caspases.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Biphenyl Compounds
/
Down-Regulation
/
Up-Regulation
/
Apoptosis
/
Lignans
/
HL-60 Cells
/
Proto-Oncogene Proteins c-bcl-2
/
Caspases
/
Cell Proliferation
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2016
Type:
Article
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