Antiapoptotic Effect of the Leukemia Associated Gene MLAA-34 in HeLa Cells / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 363-368, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-360084
ABSTRACT
<p><b>OBJECTIVE</b>To study the antiapoptotic effect of leukemia-associated gene MLAA-34 in HeLa cells.</p><p><b>METHODS</b>The MLAA-34 recombinant lentiviral expression vector was constructed, and the stably transfected HeLa cell line with high expression of MLAA-34 was set up; As(2)O(3) was used to induce apoptosis; the MTT assay, colony formation test and flow cytometry were used to detect the ability of cell proliferation, colong formation, apoptosis and cell cycle changes respectively.</p><p><b>RESULTS</b>After treatment with As(2)O(3), the survival rate of HeLa cells with MLAA-34 overexpression was significantly higher than that of the control cells, and the colony formation ability of MLAA-34 significantly increased, and the high expression of MLAA-34 gene significantly decreased the apoptosis rate of HeLa cells, and decreased the proportion of G(2)/M phase cells.</p><p><b>CONCLUSION</b>The leukemia-associated gene MLAA-34 has been comfirmed to show antiapoptotic effect in HeLa cells which are induced by As(2)O(3).</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oxides
/
Arsenicals
/
HeLa Cells
/
Transfection
/
Cell Cycle
/
Apoptosis
/
Lentivirus
/
Cell Proliferation
/
Apoptosis Regulatory Proteins
/
Genetics
Limits:
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2016
Type:
Article
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