Application of Combined Detection of Fusion Gene and BIOMED-2 Standardized Ig Gene Rearrangement System in Childhood B-cell Acute Lymphoblastic Leukemia / 中国实验血液学杂志
Journal of Experimental Hematology
;
(6): 352-357, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-360086
ABSTRACT
<p><b>OBJECTIVE</b>To explore the application of combined detection of fusion gene and BIOMED-2 standardized immunoglobulin (Ig) gene rearrangement system in diagnosis and treatment of children with acute lymphoblastic leukemia (ALL).</p><p><b>METHODS</b>Multiplex-PCR amplifications and RQ-PCR of RNA/DNA were performed using ALL fusion gene detection kit and BIOMED-2 primer. The Ig gene rearrangements were analyzed by using PCR fragment analysis system.</p><p><b>RESULTS</b>Out of 251 children with B-ALL, 77 cases were TEL-AML1(+) , 28 cases were E2A-PBX1(+) , 10 cases were MLL-AF4(+) , 11 cases were BCR-ABL(+) , the total positive rate was 50.2%, 82.5% showed IgH VH-JH rearrangement, 53.4% showed IgK rearrangement. The positive rate of combined detection of fusion gene and gene rearrangement was 99%. E2A-PBX1(+) and MLL-AF4(+) with IgK(+) gene rearrangement group was compared with negative control group, the difference was statistically significant (P < 0.001 or P = 0.005); 105 ALL fusion gene positive cases had been detected by fluorescence in situ hybridization (FISH) simultaneously, the accordance rate of fusion gene and FISH was more than 94%.</p><p><b>CONCLUSION</b>The combined detection of ALL fusion gene and BIOMED-2 standardized clonality analysis system can improve the positive detected rate of B-ALL dramatically, and make the grouping of disease prognosis more accurately; this combined detection is a more faster and sensitive method than FISH.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oncogene Proteins, Fusion
/
Fusion Proteins, bcr-abl
/
In Situ Hybridization, Fluorescence
/
DNA Primers
/
Diagnosis
/
Core Binding Factor Alpha 2 Subunit
/
Precursor Cell Lymphoblastic Leukemia-Lymphoma
/
Multiplex Polymerase Chain Reaction
/
V(D)J Recombination
/
Genetics
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Child
/
Humans
Language:
Chinese
Journal:
Journal of Experimental Hematology
Year:
2016
Type:
Article
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