µ-opioid receptors in the central nucleus of the amygdala mediate sodium intake in rats / 南方医科大学学报

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the opioidergic mechanism of the central nucleus of the amygdala (CeA) for regulating sodium appetite in rats.</p><p><b>METHDOS</b>Using the elaborate invasive cerebral cannulation and brain microinjection method, we observed the effects of bilateral intra-CeA injections of DAMGO (a selective µ-opioid receptor agonist) and CTAP (a highly selective µ-opioid receptor antagonist), either alone or in combination, on NaCl solution (0.3 mol/L) and water intake by rats in different models of Naingestion.</p><p><b>RESULTS</b>In the two-bottle tests, bilateral injections of DAMGO at 1, 2, and 4 nmol into the CeA induced a dose-related increase of NaCl and water intake in rats treated with water deprivation with partial rehydration (WD-PR), and pretreatment with 0.5, 1, and 2 nmol CTAP injected into the CeA significantly suppressed DAMGO-induced NaCl and water intake in a dose-dependent manner in the one-bottle tests, bilateral injections of DAMGO (2 noml) into the CeA had no effect on water intake of the rats. In rats with subcutaneous injection of furosemide (FURO) combined with captopril (CAP) (FURO+CAP), bilateral intra-CeA injections of DAMGO (2 nmol) caused increased NaCl and water intake in the two-bottle tests, but such effects were suppressed by pretreatment with CTAP injection into the CeA; in the one-bottle tests, bilateral intra-CeA injections of DAMGO had no effect on water intake of the rats.</p><p><b>CONCLUSION</b>µ-opioid receptors in the CeA are involved in the excitatory regulation of sodium appetite to mediate sodium intake. µ-opioid receptor antagonists are expected to be targets for developing inhibitors of sodium appetite.</p>