Depletion of Mitochondrial DNA Stabilizes C1qTNF-Related Protein 6 mRNA in Muscle Cells
Journal of Korean Medical Science
;
: 465-470, 2012.
Article
in English
| WPRIM
| ID: wpr-36039
ABSTRACT
Mutation and reduction of mitochondrial DNA (mtDNA) have been suggested as factors in the pathogenesis of several metabolic diseases. Recently, we demonstrated that C1qTNF-related protein-6 (CTRP6) is involved in fatty acid metabolism in muscle cells. In this study, we showed that expression of CTRP6 was up-regulated in mtDNA-depleted C2C12 cells, which displayed a marked decrease in cellular mtDNA and ATP content. Replacement of mtDNA normalized the expression level of CTRP6 similar to that in normal C2C12 cells, indicating that CTRP6 expression was up-regulated by mtDNA depletion. However, CTRP6 promoter activity remained unchanged in mtDNA-depleted cells. We also found that mtDNA depletion inhibited decay of CTRP6 mRNA. Taken together, mtDNA depletion induces an increase in CTRP6 expression by increasing mRNA stability.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
DNA, Mitochondrial
/
RNA, Messenger
/
Up-Regulation
/
Cell Line
/
Promoter Regions, Genetic
/
RNA Stability
/
Adiponectin
Limits:
Animals
Language:
English
Journal:
Journal of Korean Medical Science
Year:
2012
Type:
Article
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