Regulatory effect of procyanidins on the expressions of interferon-gamma, interleukin-12 and transcription factor T-bet mRNA in peripheral blood mononuclear cell of patients with alopecia areata / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 900-902, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-360528
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the regulatory effect of procyanidins (PC) on the expressions of Th1 type cytokines (IFN-gamma and IL-12) and the transcription factor T-bet mRNA in peripheral blood mononuclear cell (PBMC) in patients with alopecia areata (AA).</p><p><b>METHODS</b>Above-mentioned expressions were detected using RT-PCR technique in 20 AA patients (8 of mild and 12 of severe degree) and 10 healthy subjects after phytoaemagglutinin (PHA) or PHA + PC stimulation.</p><p><b>RESULTS</b>After being stimulated by PHA and PHA + PC, in patients with severe AA, the expression of T-bet mRNA was 0.581 +/- 0.148 and 0.419 +/- 0.113 respectively; that of IFN-gamma mRNA, 0.689 +/- 0.219 and 0.430 +/- 0.162; and that of IL-12 mRNA, 0.198 +/- 0.056 and 0.136 +/- 0.035, respectively. As compared with those before stimulation, the respective difference was statistically significant (P < 0.05).</p><p><b>CONCLUSIONS</b>PC can inhibit the expressions of Th1 type cytokines and transcription factor T-bet in PBMC of severe AA patients, and reverse the Th1 responses. The authors offered that it maybe part of the acting mechanism of pine needle for treatment of AA.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Leukocytes, Mononuclear
/
Case-Control Studies
/
Gene Expression
/
Cells, Cultured
/
Interferon-gamma
/
Interleukin-12
/
T-Box Domain Proteins
/
Proanthocyanidins
/
Drug Therapy
/
Allergy and Immunology
Type of study:
Observational study
Limits:
Adolescent
/
Adult
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Integrated Traditional and Western Medicine
Year:
2008
Type:
Article
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