Effect of human cytomegalovirus infection on nerve growth factor expression in human glioma U251 cells / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
;
(12): 354-358, 2009.
Article
in English
| WPRIM
| ID: wpr-360654
ABSTRACT
<p><b>OBJECTIVE</b>To explore the change of endogenic nerve growth factor (NGF) expression in human glioma cells infected with human cytomegalovirus (HCMV).</p><p><b>METHODS</b>U251 cells were cultured in RPMI 1640 culture medium and infected with HCMV AD169 strain in vitro to establish a cell model of viral infection. Morphologic changes of U251 cells were observed under inverted microscope before and after infection with HCMV. Expression of NGF gene and protein of cells was detected by RT-PCR and Western blotting before and after infection with HCMV.</p><p><b>RESULTS</b>The cytopathic effects of HCMV-infected cells appeared on day 5 after infection. However, differential NGF expression was evident on day 7. NGF expression was decreased significantly in U251 cells on day 7 after infection in comparison with control group (P < 0.05).</p><p><b>CONCLUSION</b>HCMV can down-regulate endogenous NGF levels in human glioma cell line U251.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Physiology
/
Virology
/
RNA, Messenger
/
Gene Expression Regulation, Neoplastic
/
Reproducibility of Results
/
Fluorescent Antibody Technique
/
Actins
/
Cytomegalovirus Infections
/
Reverse Transcriptase Polymerase Chain Reaction
Type of study:
Prognostic study
Limits:
Humans
Language:
English
Journal:
Biomedical and Environmental Sciences
Year:
2009
Type:
Article
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