Effect of hepatitis B virus X gene on the expression of spastic paraplegia 21 / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 920-923, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-360797
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of hepatitis B virus(HBV) X gene on the expression of SPG21.</p><p><b>METHODS</b>The expressions of SPG21 mRNA and protein in HepG2 and HepG2.2.15 cells were tested by RT-PCR and western blot. HepG2 cells were co-transfected with reporter plasmid pGL3-SPG21 and plasmids carrying individual genes of HBV, the luciferase activity was measured and the expressions of SPG21 were detected by RT-PCR and western blot.</p><p><b>RESULTS</b>The expressions of SPG21 mRNA and protein were higher in HepG2.2.15 cells than in HepG2 cells (0.36+/-0.06 vs 0.21+/-0.05, P value is less than 0.05). The activity of SPG21 in HepG2 cells transfected with pCMV-X was higher (875+/-27 vs 67+/-12, P value is less than 0.01) as compared to blank control group (transfected with pCMV-tag2B). HBV X gene enhanced SPG21 gene promoter activity, SPG21 mRNA expression and SPG21 protein production in HepG2 cells in a dose-dependent manner.</p><p><b>CONCLUSION</b>HBV X gene can specially activate SPG21 expression.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
DNA, Viral
/
RNA, Messenger
/
Transfection
/
Trans-Activators
/
Hepatitis B virus
/
Adaptor Proteins, Signal Transducing
/
Hep G2 Cells
/
Genetics
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2010
Type:
Article
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