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Cross-talk between PI3K/Akt and MEK/ERK pathways regulates human hepatocellular carcinoma cell cycle progression under endoplasmic reticulum stress / 中华肝脏病杂志
Chinese Journal of Hepatology ; (12): 909-914, 2010.
Article in Chinese | WPRIM | ID: wpr-360799
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the cross-talk between the PI3K/Akt and MEK/ERK pathways and its role in cell cycle regulation under endoplasmic reticulum stress in human hepatocellular carcinoma cells.</p><p><b>METHODS</b>PI3K inhibitor LY294002 and MEK inhibitor U0126 were used to block the PI3K/Akt and MEK/ERK pathways respectively, and constitutively activated Akt mutant construct was used to activate the PI3K/Akt pathway. Western blot was used to study the potential cross-talk between the PI3K/Akt and MEK/ERK pathways under endoplasmic reticulum stress in human hepatocellular carcinoma cells. the role of the cross-talk between the PI3K/Akt and MEK/ERK pathways in cell cycle regulation was investigated by using propidium iodide staining.</p><p><b>RESULTS</b>LY294002 not only blocked Akt activation efficiently but also increased ERK phosphorylation markedly under endoplasmic reticulum stress in SMMC-7721 and Hep3B cells. Furthermore, myr-Akt inhibited endoplasmic reticulum stress-mediated ERK phosphorylation. In contrast, MEK inhibitor U0126 had no effect on endoplasmic reticulum stress-induced Akt activation. It is notable that both myr-Akt overexpression and MEK inhibitor U0126 inhibited endoplasmic reticulum stress-induced G0/G1 phase arrest in SMMC-7721 cells.</p><p><b>CONCLUSION</b>Endoplasmic reticulum stress-induced Akt activation is mediated through PI3K and the PI3K/Akt pathway inactivation is involved in increased ERK activity in human hepatocellular carcinoma cells. The cross-talk between the PI3K/Akt and MEK/ERK cascades plays an important role in endoplasmic reticulum stress-induced human hepatocellular carcinoma cell cycle arrest.</p>
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Phosphorylation / Butadienes / Signal Transduction / Cell Cycle / Morpholines / Chromones / Carcinoma, Hepatocellular / Mitogen-Activated Protein Kinase Kinases / Cell Line, Tumor Limits: Humans Language: Chinese Journal: Chinese Journal of Hepatology Year: 2010 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Phosphorylation / Butadienes / Signal Transduction / Cell Cycle / Morpholines / Chromones / Carcinoma, Hepatocellular / Mitogen-Activated Protein Kinase Kinases / Cell Line, Tumor Limits: Humans Language: Chinese Journal: Chinese Journal of Hepatology Year: 2010 Type: Article