Effects of simvastatin on the proliferation of HepG2 cells / 中华肝脏病杂志
Chinese Journal of Hepatology
;
(12): 751-753, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-360848
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects of simvastatin on the proliferation, cell cycle and expression of cyclin-dependent kinase inhibitor p21 protein in human hepatocellular carcinoma (HepG2) cells in vitro.</p><p><b>METHODS</b>HepG2 cells were administrated with simvastatin. Proliferation of the cells was detected by MTT assay, cell cycle was measured by flowcytometry and the cyclin-dependent kinase inhibitor p21 protein expression was detected by immunocytochemistry. The results were evaluated by factorial design and one-way analysis of variance.</p><p><b>RESULTS</b>Simvastatin inhibited HepG2 cells growth in vitro (F(concentration) = 1264, P value less than 0.001; F(time) = 17.466, P value less than 0.001; F(concentration*time) = 35.053, P value less than 0.001) and could arrest HepG2 cells in G0/G1 phase of cell cycle. However, apoptosis of HepG2 cells was not obvious. Simvastatin could also increase cyclin-dependent kinase inhibitor p21 protein expression (F = 512.133, P value less than 0.001).</p><p><b>CONCLUSION</b>Simvastatin can inhibit the growth of HepG2 cells in vitro, which may be explained by its effects of enhancing cyclin-dependent kinase inhibitor p21 protein expression and arresting HepG2 cells at G0/G1 phase of cell cycle.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Cell Cycle
/
Carcinoma, Hepatocellular
/
Simvastatin
/
Cell Proliferation
/
Cyclin-Dependent Kinase Inhibitor p21
/
Hep G2 Cells
/
Liver Neoplasms
/
Metabolism
Limits:
Humans
Language:
Chinese
Journal:
Chinese Journal of Hepatology
Year:
2010
Type:
Article
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