The significance of the expression of cyclooxygenase-2 in bladder transitional cell carcinoma tissues / 中华外科杂志
Chinese Journal of Surgery
;
(12): 1104-1107, 2004.
Article
in Chinese
| WPRIM
| ID: wpr-360921
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of cyclooxygenase-2 (COX-2) in bladder transitional cell carcinoma tissues, and understand its clinical significance.</p><p><b>METHODS</b>Reversal transcription polymerase chain reaction (RT-PCR) was used to assess the expression of COX-2 mRNA in 52 cases of bladder transitional cell carcinoma tissues and 17 cases of normal bladder tissues far from neoplasm; Western blot was used to assess the expression of COX-2 protein in 49 cases of bladder cancerous tissues and 17 cases of normal tissues.</p><p><b>RESULTS</b>Positive expression of COX-2 mRNA was detected in 83% (43/52) of bladder cancer tissues and in 29% (5/17) of normal tissues by RT-PCR and there was significant difference in expression of COX-2 mRNA between cancer tissues and normal tissues. Western blot analysis showed that expression of COX-2 protein was correlation with the stage and grade of cancer.</p><p><b>CONCLUSION</b>COX-2 is overexpressed in bladder transitional cell carcinoma. COX-2 maybe play a certain role in carcinogenesis and progression of bladder cancer and turn into a useful target of chemoprevention of bladder cancer.</p>
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Urinary Bladder
/
Urinary Bladder Neoplasms
/
RNA, Messenger
/
Carcinoma, Transitional Cell
/
Blotting, Western
/
Reverse Transcriptase Polymerase Chain Reaction
/
Cyclooxygenase 2
/
Genetics
/
Neoplasm Staging
Limits:
Adult
/
Aged
/
Aged80
/
Female
/
Humans
/
Male
Language:
Chinese
Journal:
Chinese Journal of Surgery
Year:
2004
Type:
Article
Similar
MEDLINE
...
LILACS
LIS