The Effect of Heavy Metals on Nicotinamide \it{N}-methyltransferase Activity \it{In Vitro} Relating to Parkinson\rqs Disease
Environmental Health and Preventive Medicine
;
: 180-183, 2005.
Article
in Japanese
| WPRIM
| ID: wpr-361409
ABSTRACT
Objective:
The aims of this study were to determine the effects of heavy metals such as manganese on nicotinamide N-methyltransferase (EC 2.1.1.1) (NNMT) activity and to consider the possibility of involvement of NNMT activation in the pathogenesis of heavy metal induced Parkinson’s disease.Methods:
NNMT activity in supernatants separated from brain, liver and kidney homogenates of 5 elderly male Wistar rats by centrifugation were measured by high performance liquid chromatography system with fluorescence. NNMT activity under the conditon of 0.5 or 5.0 mM Mn2+, Fe2+, Cu2+ or Cd2+ was compared with control (no metal ion existence).Results:
NNMT activities in rat brain, liver and kidneys were significantly decreased by Cu2+, and those in the liver and kidneys were significantly decreased by Cd2+. Mn2+ reduced NNMT activity only in the liver. Fe2+ had no effect on NNMT activity.Conclusions:
No metal increased NNMT activity in this study, contrary to our hypothesis. Further study is needed to clarify the reason why the effects of Mn2+ and Fe2+ which have a high relevance to Parkinson’s disease on NNMT activity differ from those of Cu2+ and Cd2+.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Metals, Heavy
/
Liver
Language:
Japanese
Journal:
Environmental Health and Preventive Medicine
Year:
2005
Type:
Article
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