No Immunotoxic Effect on T Cells with Di (2-Ethylhexyl) Phthalate in Male C57BL/6 Mice
Environmental Health and Preventive Medicine
;
: 59-63, 2003.
Article
in Japanese
| WPRIM
| ID: wpr-361480
ABSTRACT
Objectives:
To clarify whether di (2-ethylhexyl) phthalate (DEHP) has immunotoxic effects on both the expression of surface molecules (CD3, CD4, CD8 and CD28) on T cells of the thymus and spleen in male C57BL/6 mice.Methods:
Animals were orally administered a 0.1% or 0.2% DEHP-containing diet for 10 or 20 days. Dietary corn oil was used as the vehicle for DEHP in preparing the diet.Results:
Significant hepatic hypertrophy was clearly observed in the DEHP-exposed groups, while no atrophy was seen in the thymus or spleen in any treatment groups. In the thymus and spleen, no variation in the proportions of both T cells expressing CD3, CD4 and CD8 was shown with cytometry analysis. The surface expression of CD3, CD4, CD8 and CD28 on both T cells was also invariable in all analyzed stages of thymic differentiation and in the spleen. No effect of DEHP on mitogenesis was shown in the splenic T cells with an in vitro [3H]-thymidine-incorporation technique.Conclusions:
DEHP is probably not an immunosuppressor, particularly for T cells.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Spleen
/
T-Lymphocytes
/
CD3 Complex
/
Diethylhexyl Phthalate
Language:
Japanese
Journal:
Environmental Health and Preventive Medicine
Year:
2003
Type:
Article
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