A DIGE PROTEOMIC ANALYSIS FOR LOW-INTENSITY EXERCISE-TRAINED RAT SKELETAL MUSCLE / 体力科学

ABSTRACT

<b>Background:</b> Exercise training induces various adaptations in skeletal muscles. However, the mechanisms remain unclear. <b>Purpose:</b> Therefore, we conducted 2D-DIGE proteomic analysis, which has not yet been used for elucidating adaptations of skeletal muscle after low-intensity exercise training (LIT). <b>Methods:</b> For five days, rats performed LIT, which consisted of two 3-h swimming exercise with45-m rest between the exercise bouts. 2D-DIGE analysis was conducted on epitrochlearis muscles excised eighteen hours after the final training exercise. <b>Results:</b> Proteomic profiling revealed that, out of 681 detected and matched spots, 22 proteins exhibited changed expression by LIT compared with sedentary rats. All proteins were identified by MALDI-TOF/MS. <b>Conclusion:</b> The proteomic 2D-DIGE analysis following LIT identified expressions of skeletal muscle proteins, includingATPsynα, UQCRC1, dihydrolipoamide dehydrogenase, dihydrolipoamide acetyltransferase, that were not previously reported to change their expressions after exercise-training.