Role of miR-511 in the Regulation of OATP1B1 Expression by Free Fatty Acid
Biomolecules & Therapeutics
;
: 400-406, 2015.
Article
in English
| WPRIM
| ID: wpr-36721
ABSTRACT
MicroRNAs (miRNAs) are a family of non-coding RNA that are able to adjust the expression of many proteins, including ATP-binding cassette transporter and organic cation transporter. We sought to evaluate the effect of miR-511 on the regulation of OATP1B1 expression by free fatty acids. When using free fatty acids to stimulate Chang liver cells, we found that the expression of miR-511 increased significantly while the expression of OATP1B1 decreased. We also proved that SLCO1B1 is the target gene of miR-511 with a bioinformatics analysis and using the dual luciferase reporter assay. Furthermore, the expressions of SLCO1B1 and OATP1B1 decreased if transfecting Chang liver cells with miR-511, but did not increase when transfecting the inhibitors of miR-511 into steatosis cells. Our study indicates that miR-511 may play an important role in the regulation of OATP1B1 expression by free fatty acids.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Computational Biology
/
RNA, Untranslated
/
MicroRNAs
/
Fatty Acids, Nonesterified
/
Liver
/
Luciferases
Limits:
Humans
Language:
English
Journal:
Biomolecules & Therapeutics
Year:
2015
Type:
Article
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