Compound K, a Metabolite of Ginsenosides, Attenuates Collagen-induced Arthritis in Mice
Journal of Rheumatic Diseases
;
: 154-166, 2015.
Article
in English
| WPRIM
| ID: wpr-36847
ABSTRACT
OBJECTIVE:
Although several ginsenosides have been reported to have anti-arthritic activity, few in vivo studies of the anti-arthritic effects of compound K (CK), a major metabolite of ginsenosides, have been conducted. Therefore, we investigated the preventative and therapeutic effects of CK on collagen-induced arthritis (CIA).METHODS:
CK was administered to CIA mice preventively and therapeutically and post-treatment bone microarchitectural characteristics, histopathological changes, and serum levels of anti-collagen antibodies, tumor necrosis factor-alpha, and interleukin (IL)-17 were investigated. We also examined cytokine production by type II collagen (CII)-stimulated splenocytes and mRNA expression of matrix metalloproteinases (MMPs), tissue inhibitors of metalloproteinase (TIMP)-1, receptor activator of nuclear factor-kappaB ligand (RANKL), and osteoprotegerin (OPG) in the joint tissues.RESULTS:
CK reduced the severity of CIA preventively and therapeutically (all p<0.05). Additionally, CK dose-dependently decreased histopathological signs of arthritis and improved microarchitectural characteristics (all p<0.05) at 10 to 20 mg/kg/d in CIA mice. CK treatment significantly decreased the serum levels of anti-CII immunoglobulin G (p<0.01) and the secretion of interferon-gamma and IL-2 from stimulated splenocytes (all p<0.05). Furthermore, MMP-3/TIMP-1 and RANKL/OPG ratios were suppressed in CK treated mice (all p<0.01).CONCLUSION:
CK attenuated CIA via suppression of the humoral immune response and modulation of joint-destructive mediators. These results suggest that CK has therapeutic potential in rheumatoid arthritis.
Full text:
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Index:
WPRIM (Western Pacific)
Main subject:
Arthritis
/
Arthritis, Experimental
/
Arthritis, Rheumatoid
/
Immunoglobulin G
/
RNA, Messenger
/
Interleukins
/
Interferon-gamma
/
Interleukin-2
/
Matrix Metalloproteinases
/
Collagen Type II
Limits:
Animals
Language:
English
Journal:
Journal of Rheumatic Diseases
Year:
2015
Type:
Article
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