(31)P MR Spectroscopic Measurement of Intracellular pH in Normal Human Hearts
Journal of the Korean Radiological Society
;
: 455-460, 2002.
Article
in English
| WPRIM
| ID: wpr-36870
ABSTRACT
PURPOSE:
To assess the usefulness of intracellular pH (pHi), calculated by determining the shift of a high-energy metabolite such as inorganic phosphate (Pi) or gamma-ATP after performing MRS with ECG-gated two-dimensional 31P CSI (chemical shift imaging), as a parameter for the overall state of the intracellular milieu. MATERIALS ANDMETHODS:
Proton decoupled 31P CSI was performed on a 1.5-T scanner using a (1)H-(31)P dual-tuned surface coil. Cardiac MRS data were obtained from eight normal volunteers aged 24-32 years with no history of heart disease. From the spectra obtained from several regions of the heart, peak position and peak area were estimated. The metabolic ratios of alpha-, beta-, gamma-ATP, PCr, Pi, phosphodiester and diphosphoglycerate were calculated, and pHi was estimated from the chemical shift of Pi and gamma-ATP resonance. We then compared the data for the anterior myocardium with those previously published.RESULTS:
The major phosphorous metabolites identified in these human hearts were as follows PCr, at -0.1 to +0.1 ppm; three phosphate peaks from ATP, with a chemical shift centered at about -2.7 ppm (gamma-ATP), -7.8 ppm (alpha-ATP), and -16.3 ppm (beta-ATP); and phosphodiester (PDE) at 2-3 ppm, inorganic phosphate (Pi) at 4.5-5.4 ppm, and diphosphoglycerate (DPG) at 5.4-6.3 ppm. The PCr/beta-ATP ratio was 2.20+/-0.17 and the PDE/beta-ATP ratio, 1.04+/-0.09. pHi readings were 7.31+/-0.23 (calculated by the shift of Pi) and 6.81+/-0.20 (calculated by the shift of gamma-ATP). Pi/PCR was 0.539, a ratio higher than that mentioned in previously published reports.CONCLUSION:
The measurement of intracelluar metabolism was affected by various kinds of factors. We believe, however, that pHi readings indicate the overall state of the cardiac intracelluar milieu. An unexpected pHi readings, seen at MRS, may reflect errors in the MR procedure itself and, or in the alanytical method.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Protons
/
Reading
/
Adenosine Triphosphate
/
Polymerase Chain Reaction
/
Healthy Volunteers
/
Heart
/
Heart Diseases
/
Hydrogen-Ion Concentration
/
Metabolism
/
Myocardium
Type of study:
Prognostic study
Limits:
Humans
Language:
English
Journal:
Journal of the Korean Radiological Society
Year:
2002
Type:
Article
Similar
MEDLINE
...
LILACS
LIS