EVALUATION OF POST EXERCISE PROTEINURIA BY THE URINARY ENZYME ACTIVITY / 体力科学

ABSTRACT

For revealing the post exercise proteinuria, the variation of leucine aminopeptidase (LAP) and γ-glutamyltranspeptidase (γ-GTP) activities were observed to know the fluctuation of the substances excreted in the urine and the composition of the urinary protein in 4 college athletes aging from 19 to 21 years before and after 10-mile road race.<BR>The results obtained are as follows<BR>1) During the race and in the early stage of recovery the excretion of creatinine, uric acid, and urea-nitrogen decreased, and in this term clearance in the kidney reduced. More than 60 minutes after the end of the road race, the excretion of these substances returned to the level before the race.<BR>2) The urinary pH value began to increase just after the race, became alkaline at pH more than 7 and less than 8 in all the subjects 30 to 60 minutes after the race, 90 to 120 minutes after the race it returned to level obtained before the race.<BR>3) In the term when the excretion of the substances decreased, as described in the above 1), protein was much excreted, and it gradually decreased in the urine collected 60 to 90 minutes and later after the race, but the level of the excreted protein was still higher than that obtained before the race.<BR>4) The Albumin/Globulin ratio of the urinary protein was low at rest, but in the early stage of recovery it was higher than 1, but in the later stage of recovery it decreased.<BR>5) LAP and γ-GTP activities were also high in the term when protein was much excreted, but gradually decreased following the progress of recovery. LAP activity in the urine collected 120 to 150 minutes after the race recovered nearly to the level before the race, but γ-GTP remained still at a rather high level, and indicated the difference in the composition of the post exercise proteinuria.<BR>6) It was estimated that the post exercise proteinuria would include not only the leaked plasma component caused by increase of the permeability but also some part derived from the renal tissue.