Analysis of human urinary proteins after physical exercise by two-dimensional electrophoresis under non-denaturing conditions / 体力科学
Japanese Journal of Physical Fitness and Sports Medicine
;
: 138-144, 1993.
Article
in Japanese
| WPRIM
| ID: wpr-371608
ABSTRACT
A study was conducted to investigate the changes in human urinary proteins caused by physical exercise. Nine subjects (male middle-distance runners, 19-21 years) were loaded physical exercise with a 10km-run, and quantitative and qualitative changes in urinary proteins were examined using various techniques including polyacrylamide gel electrophoresis, immunoelectrophoresis and two-dimensional electrophoresis. Total protein concentrations in urine from all subjects before exercise were very low (0.68-2.56mg/d<I>l</I>), but those were increased remarkably after exercise. Few proteins except for albumin were detected in urine before run by polyacrylamide gel electrophoresis and immunoelectrophoresis, however, a few proteins were observed after exercise in addition to a marked increase of albumin. Several proteins which were not observed in serum could also be detected in urine after exercise by two-dimensional electrophoresis under non-denaturing condition. It is considered that two-dimensional electrophoresis is a very powerful technique for analyzing dilute and complicated protein mixture in biological fluids such as urine.
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Qualitative research
Language:
Japanese
Journal:
Japanese Journal of Physical Fitness and Sports Medicine
Year:
1993
Type:
Article
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