The Effects of Tranilast gamma on the Cellular Proliferation and alpha-Smooth Muscle Actin Expression in the Cultured Keratocytes of Rabbit
Journal of the Korean Ophthalmological Society
;
: 668-674, 2004.
Article
in Korean
| WPRIM
| ID: wpr-37401
ABSTRACT
PURPOSE:
To evaluate the effects of Tranilast gamma on the cellular proliferation and expression of alpha-smooth muscle actin on the cultured keratocytes of the rabbit that influence the formation of corneal scar and haze in vitro.METHODS:
After the keratocytes of the rabbit were cultured, they were exposed to Tranilast gamma (N-(3, 4-dimethoxycinnamoyl) anthranilic acid) 0.05, 0.1, 0.2, 0.4, 0.8, 1.0, 1.6 mg/ml with DMEM used as a control. MTT was used to measure the metabolic activity, and the Western blot assay was performed to confirm the alpha-smooth muscle actin expression, which was expressed with time by Tranilast gamma treatment.RESULTS:
Higher the concentration and longer the exposure time of Tranilast gamma, the more the inhibition of the cellular proliferation. LD50 concentration was about 0.4 mg/ml in the exposure time of 24 hours and 0.2 mg/ml in the exposure time of over 48 hours(P<0.05). There was a decrease in response of alpha-smooth muscle actin expression with increasing concentrations of Tranilast gamma relative to the control.CONCLUSIONS:
Tranilast gamma trends to have an inhibitory effect on the cellular proliferation and induction of alpha-smooth muscle actin expression. Tranilast gamma may inhibit excessive haze of the cornea and inhibit scar formation after the corneal wound healing and photorefractive surgery in the cornea.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Wound Healing
/
Blotting, Western
/
Actins
/
Cicatrix
/
Cornea
/
Cell Proliferation
/
Lethal Dose 50
Language:
Korean
Journal:
Journal of the Korean Ophthalmological Society
Year:
2004
Type:
Article
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