Mutations in the pre-core region of hepatitis B virus DNA in patients with chronic liver diseases
Yonsei Medical Journal
;
: 158-165, 1993.
Article
in English
| WPRIM
| ID: wpr-37568
ABSTRACT
To investigate the prevalence of point mutation in the pre-core (pre-C) region of hepatitis B virus (HBV) DNA, we performed dot blot hybridization and sequencing of enzymatically amplified HBV DNA from the sera of 25 patients with HBeAg-positive and 32 patients with HBeAg-negative chronic liver diseases. The pre-C region of HBV DNA was successfully amplified by polymerase chain reaction (PCR) from 55 (96.5%) of 57 sera. According to the status of serum HBeAg, HBV DNA was amplified from all 25 sera of HBeAg-positive patients and 30 (93.8%) of 32 sera of HBeAg-negative patients. All amplified DNA from the sera of 25 patients with HBeAg-positive and that from 28 (93.3%) of 30 patients with HBeAg-negative chronic liver diseases hybridized with the wild type probe. In addition, that from 5 (20.0%) among 25 patients with HBeAg-positive and 16 (53.3%) among 30 patients with HBeAg-negative chronic liver diseases hybridized also with the mutant type probe. These results suggest that the prevalence of point mutation in the pre-C region of HBV DNA is relatively high in patients with HBeAg-negative chronic liver diseases and further study is mandatory to identify the significance of this mutation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
DNA, Viral
/
Molecular Sequence Data
/
Base Sequence
/
Molecular Probes
/
Hepatitis B virus
/
Chronic Disease
/
Liver Diseases
/
Mutation
Limits:
Humans
Language:
English
Journal:
Yonsei Medical Journal
Year:
1993
Type:
Article
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