Anti-human Cytomegalovirus Activity of Hot Water Extract from Sasa senanensis Rehder / 日本補完代替医療学会誌

ABSTRACT

<i>Objective</i> Anti-Human cytomegalovirus (HCMV) activity of Kumazasa (<i>Sasa senanensis</i> Rehder) extract were investigated in the human embryonic lung (HEL) fibroblast cell.<br> <i>Method</i> This study examined the effect of hot water extract from <i>Sasa senanensis </i>Rehder on HCMV replication, viral-specific major immediate early (IE) gene expression, and protein synthesis.<br> <i>Results</i> Treatment of HCMV-infected HEL cells with that <i>Sasa senanensis</i> Rehder extract inhibited the cytopathic effect and viral production in a dose-dependent manner. Further, real-time RT-PCR assay and Western blotting analysis revealed that <i>Sasa senanensis</i> Rehder extract decreased expression of the HCMV IE gene in virus-infected HEL cell.<br> <i>Conclusion</i> These results suggest that <i>Sasa senanensis</i> Rehder extract have anti-HCMV activity by inhibitting of IE gene expression.<br>