Safety Evaluation of Fucoidan from <i>Kjellmaniella Crassiforia</i> and Extract from <i>Hypsizigus Marmoreus</i>: Influence on the Activities and Gene Expressions of Rat Hepatic CYPs / 日本補完代替医療学会誌

ABSTRACT

<b>Object</b> Gagome kombu (<i>Kjellmaniella cracciforia</i>) is the edible brown seaweed and contains fucoidan, a sulfated polysaccharide, abundantly. Bunashimeji (<i>Hypsizigus marmoreus</i>) is the popular Japanese mushrooms and contains polyterpenes as the bitter substance. Previously, we investigated the bioactive functions (e.g. anti-tumor action) and the safety of fucoidan from Gagome kombu (GKF) and the extract from Bunashimeji (KTE Kinoko terpene extract). In this study, we evaluate the influence of GKF and KTE on hepatic cytochrome P450 (CYP).<br> <b>Methods:</b> Male SD rats were divided into three groups (n = 5). 2,000 mg/kg of GKF and KTE were given orally once daily for 4 days.<br> <b>Result:</b> There were no difference in activities and mRNA expressions of hepatic CYPs (CYP2C11, CYP2D, CYP2E1 and CYP3A1) among all groups.<br> <b>Conclusion:</b> These results indicated GKF and KTE did not influence the rat hepatic CYPs.<br>