Urinary Levels of 8-Iso-Prostaglandin F2alpha and 8-Hydroxydeoxyguanine as Markers of Oxidative Stress in Patients With Coronary Artery Disease

ABSTRACT

BACKGROUND AND OBJECTIVES: The objective of this study was to determine if urinary levels of 8-iso-prostaglandin F2alpha (8-iso-PGF2alpha) and 8-hydroxydeoxyguanine (8-OHdG) could be used as markers of the oxidative stress in significant coronary artery disease (CAD). SUBJECTS AND METHODS: We conducted a case-control study in 104 subjects assessed by coronary angiography with the following diagnoses 35 consecutive cases of significant CAD and 69 cases of non-CAD with stable angina. We compared the urinary levels of 8-iso-PGF 2alpha and 8-OHdG, as measured by immunoassay between the 2 groups. RESULTS: History of hypertension was significantly higher and high density lipoprotein-cholesterol level significantly lower in the CAD group compared with those in the non-CAD group. Median levels of 8-iso-PGF2alpha were significantly higher in the CAD group compared with the non-CAD group (9.2 vs. 6.0 ng/mg, p=0.001). There were no significant differences in 8-OHdG values between the 2 groups. The odds ratio of 8-iso-PGF2alpha for CAD in the highest tertile compared with that in the lowest tertile was 7.39 (95% confidence interval; 1.71-31.91). There was no significant difference in median values of 8-iso-PGF2alpha between single- and multi-vessel CAD. CONCLUSION: Urinary 8-iso-PGF 2alpha was independently associated with significant CAD in this case-control study.