Characteristics of Serum Insulin-like Growth Factor ( IGF ) and IGF-Bindign Protein-3 during Pregnancy / 대한내분비학회지
Journal of Korean Society of Endocrinology
;
: 376-385, 1997.
Article
in Korean
| WPRIM
| ID: wpr-37798
ABSTRACT
BACKGROUND:
Pregnancy in human and rodents is associated with dramatic matemal metabolic changes. Insulin-like growth factors (IGFs) are mitogenic peptides that are essential for fetal and maternal tissue growth during pregnancy. They circulate complexed primarily with a serum IGF-binding protein (IGFBP-3) which regulates the availability of the IGFs to their specific target tissues.METHODS:
To examine the changes of IGFs and IGFB-3 during pregnancy, we measured serum total IGF-I, free IGF-I, IGF-II and IGFBP-3 by using specific radioimmunoassay, immunoradio-metric assay, western ligand blot and western immunoblot. Blood samples were obtained from 88 pregnant women between 6-40 weeks gestation.RESULTS:
While serum IGF-I levels increased up to 50% in late pregnancy, serum IGF-II levels remained unchanged. However, serum free IGF-I levels were significantly higher during pregnancy than in nonpregnancy. Western ligand blot analysis revealed that IGFBP-3 in pregnancy serum was significantly decreased at 6 weeks of gestation, continued decreased level until term, and returned to a nonpregnant level by postpartum 10 day. Serum IGFBP-3 profiles in Western immunoblot analysis revealed that 30 kDa fragments of IGFBP-3 were detectable in pregnancy serum but not in nonpregnancy serum. In contrast, serum IGFBP-3 levels using radioimmunoassay was significantly increased in late pregnancy.CONCLUSIONS:
1) serum IGF-I was significantly elevated in late pregnancy 2) serum IGF-II was not significantly changed 3) free IGF-I significantly elevated throughout gestation 4) intact IGFBP-3 was markedly reduced after 6 weeks of gestation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Peptides
/
Rodentia
/
Somatomedins
/
Insulin-Like Growth Factor I
/
Insulin-Like Growth Factor II
/
Radioimmunoassay
/
Blotting, Western
/
Insulin-Like Growth Factor Binding Protein 3
/
Pregnant Women
/
Postpartum Period
Limits:
Female
/
Humans
/
Pregnancy
Language:
Korean
Journal:
Journal of Korean Society of Endocrinology
Year:
1997
Type:
Article
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