Regulation of Steroid Thyroid Hormone Receptor 3 (TR3) mRNA Expression by Luteinizing Hormone in Human Early Luteinized Granulosa Cells / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
;
: 1736-1745, 2002.
Article
in Korean
| WPRIM
| ID: wpr-37867
ABSTRACT
OBJECTIVE:
The present study examined the gonadotropin regulation of TR3 gene expression by luteinizing hormone (LH) in cultured human luteinized granulosa cells.METHODS:
TR3 mRNA levels were detected by competitive reverse transcriptase-polymerase chain reaction (RT-PCR) method in cultured human luteinized granulosa cells collected from patients undergoing in vitro fertilization.RESULTS:
TR3 transcript was transiently induced by LH, reaching maximum levels 1 hr after stimulation, in a dose-dependent manner. LH-stimulated TR3 expression was abolished by actinomycin D, but was superinduced by cycloheximide. Treatment of luteinized granulosa cells with Rp-cAMP, an inhibitor of protein kinase A, as well as, chelerythrin, an inhibitor of protein kinase C, suppressed LH-stimulated TR3 mRNA levels. In addition, forskolin and TPA mimicked the LH action on the induction of TR3 gene, implying the role of protein kinase A and C activation.CONCLUSION:
Taken together, the present study demonstrates that TR3 gene was rapidly and transiently induced by LH in human luteinized granulosa cells. The results imply that TR3 may play a role in ovulation by initiating a cascade of ovulation-specific gene expression in response to LH.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ovulation
/
Thyroid Gland
/
Lutein
/
Receptors, Thyroid Hormone
/
Colforsin
/
Protein Kinase C
/
RNA, Messenger
/
Luteinizing Hormone
/
Fertilization in Vitro
/
Gene Expression
Limits:
Female
/
Humans
Language:
Korean
Journal:
Korean Journal of Obstetrics and Gynecology
Year:
2002
Type:
Article
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