Endothelin-1, Endothelin-2 and Endothelin-3 Induced Expression of Monocyte Chemoattractant Protein-1 in Rat Mesangial Cells / 대한신장학회잡지
Korean Journal of Nephrology
;
: 358-365, 2003.
Article
in Korean
| WPRIM
| ID: wpr-37966
ABSTRACT
BACKGROUND:
Monocyte chemoattractant protein- 1 (MCP-1) is an important mediator for monocyte/ macrophage infiltration in various inflammatory renal diseases and is produced by renal cells. In the process of renal diseases, endothelin-1 (ET-1) is known to play an active role in cell growth, inflammation and fibrosis. The aim of this study was to investigate whether three isoforms of endothelin regulate MCP-1 expression in cultured mesangial cells.METHODS:
Mesangial cells were incubated with or without various doses of ET-1, ET-2 or ET-3. To determine the monocyte chemotactic activity, chemotaxis assay was performed in modified Boyden chambers using freshly isolated human monocytes. MCP-1 mRNA expression in mesangial cells was measured by Northern blot analysis.RESULTS:
ET-1, ET-2 and ET-3 stimulated monocyte chemotactic activity released from mesangial cells in a dose-dependent manner. ET-1, ET-2 and ET-3 also stimulated MCP-1 mRNA expression in a time-dependent manner, which was seen as early as 4 hours and was maintained up to 24 hours.CONCLUSION:
These data suggest that ET-1, ET- 2 and ET-3 stimulate MCP-1 expression in mesangial cells and may contribute to the monocyte/ macrophage infiltration in inflammatory renal diseases.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Fibrosis
/
RNA, Messenger
/
Monocytes
/
Chemotaxis
/
Blotting, Northern
/
Endothelins
/
Chemokine CCL2
/
Endothelin-1
/
Endothelin-2
/
Endothelin-3
Limits:
Animals
/
Humans
Language:
Korean
Journal:
Korean Journal of Nephrology
Year:
2003
Type:
Article
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