Apoptosis of Prostate Cancer by Bax Gene Expression / 대한비뇨기과학회지
Korean Journal of Urology
;
: 916-923, 2003.
Article
in Korean
| WPRIM
| ID: wpr-38007
ABSTRACT
PURPOSE:
To evaluate the antitumor effect of the proapoptotic Bax gene in prostate cancer cells, in vitro, using a plasmid vector expressing the human Bax gene. MATERIALS ANDMETHODS:
cDNA of the human Bax gene, amplified by RT-PCR, was cloned to pCR@3.1. The expression of the cloned Bax (pCR3.1-Bax) was observed by RT-PCR and Western blot analyses. The efficacy of growth inhibition by the cloned Bax gene was tested, in vitro, on PC-3 and DU145 human prostate cancer cell lines using the MTT assay. Immunoblot analysis for the expressions of Bcl-2 and Bcl-xL were performed. Assays were also performed to evaluate the apoptosis, DNA fragmentation and CPP32.RESULTS:
The Bax protein was expressed in the parental PC-3 cells, but not in the DU-145 cells. The expressions of Bax mRNA in the transfected PC-3 and DU-145 cells had increased by 24 hr, and those of Bax protein in the transfected PC-3 and DU-145 cells had increased by 48 and 24 hr, respectively, compared with the control cell lines. The cytotoxicity of pCR3.1-Bax on PC-3 and DU-145 cells increased significantly compared with an empty vector, pCR3.1 (p<0.05, respectively). An increased cytotoxicity of the Bax-transfected cell lines was associated with enhanced apoptosis. The Bcl-2 protein was not expressed in the transfected cells, and the levels of Bcl-xL protein expression in transfected cells were no different to those in the parenteral cells. The Bax/Bcl-xL ratio was increased by the transfection of the Bax expression vector.CONCLUSIONS:
Our results show that the cloned Bax-expression plasmid vector efficiently inhibits the growth of PC-3 and DU145 human prostate cancer cell lines. These data suggest that exogenous Bax expression may have therapeutic applications in prostate cancer.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Parents
/
Plasmids
/
Prostate
/
Prostatic Neoplasms
/
RNA, Messenger
/
Transfection
/
Genetic Therapy
/
Gene Expression
/
Cell Line
/
Blotting, Western
Limits:
Humans
Language:
Korean
Journal:
Korean Journal of Urology
Year:
2003
Type:
Article
Similar
MEDLINE
...
LILACS
LIS