High-glucose up-regulates the expression of fibronectin mediated by integrin-linked kinase in renal tubular epithelial cells / 中华肾脏病杂志
Chinese Journal of Nephrology
; (12): 571-574, 2008.
Article
in Zh
| WPRIM
| ID: wpr-380100
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ABSTRACT
Objective To investigate the relationship between high-glucose-induced fibronectin(FN) expression and up-regulation of integrin-linked kinase(ILK) in human kidney tubular epithelial cells (HKC) and kidney of CD-1 mice. Methods Cultured human kidney tubular epithelial cells and streptozotocin (STZ)-indueed diabetic model of CD-1 mice were enrolled in this study.Western blot was used to detect the expression of FN and ILK.The kinase dead ILK plasmid (pCMV-kdlLK) were transferred to HKC. Results Four weeks after injection of STZ,CD-1 mice had higher blood glucose level as compared to the control [(20.3±2.7) mmol/L vs (6.1±1.4) mmol/L,P<0.01].Meanwhile,expression of FN and ILK was significantly increased in diabetic mice as compared to the control (P<0.01).There was positive correlation between the expression of FN and ILK (r=0.899,P<0.01).High-glucose could up-regulate FN and ILK expression in cultured HKC in a time- and dose-dependent manner.Blockage of ILK activation by pCMV-kdILK abrogated high-glucose-incuced FN expression in HKC. Conclusions Highglucose can induce FN expression through up-regulating ILK expression.Blockage of ILK activation abrogates this effect.
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WPRIM
Language:
Zh
Journal:
Chinese Journal of Nephrology
Year:
2008
Type:
Article