Involvement of TRPA1 in the cinnamaldehyde-induced pulpal blood flow change in the feline dental pulp
Restorative Dentistry & Endodontics
;
: 202-209, 2016.
Article
in English
| WPRIM
| ID: wpr-38031
ABSTRACT
OBJECTIVES:
The purpose of this study was to investigate the involvement of TRPA1 in the cinnamaldehyde-induced pulpal blood flow (PBF) change in the feline dental pulp. MATERIALS ANDMETHODS:
Mandibles of eight cats were immobilized and PBF was monitored with a laser Doppler flowmetry at the mandibular canine tooth. To evaluate the effect of cinnamaldehyde on PBF, cinnamaldehyde was injected into the pulp through the lingual artery at a constant rate for 60 seconds. As a control, a mixture of 70% ethanol and 30% dimethyl sulfoxide (DMSO, vehicle) was used. To evaluate the involvement of transient receptor potential ankyrin 1 (TRPA1) in PBF change, AP18, a specific TRPA1 antagonist, was applied into the pulp through the Class V dentinal cavity followed by cinnamaldehyde-administration 3 minutes later. The paired variables of experimental data were statistically analyzed using paired t-test. A p value of less than 0.05 was considered as statistically significant.RESULTS:
Administration of cinnamaldehyde (0.5 mg/kg, intra-arterial [i.a.]) induced significant increases in PBF (p 0.05), administration of cinnamaldehyde (0.5 mg/kg, i.a.) following the application of AP18 (2.5 - 3.0 mM, i.c.) resulted in an attenuation of PBF increase from the control level (p < 0.05). As a result, a TRPA1 antagonist, AP18 effectively inhibited the vasodilative effect of cinnamaldehyde (p < 0.05).CONCLUSIONS:
The result of the present study provided a functional evidence that TRPA1 is involved in the mechanism of cinnamaldehyde-induced vasodilation in the feline dental pulp.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Arteries
/
Vasodilation
/
Dimethyl Sulfoxide
/
Laser-Doppler Flowmetry
/
Ankyrins
/
Cuspid
/
Dental Pulp
/
Dentin
/
Ethanol
/
Mandible
Limits:
Animals
Language:
English
Journal:
Restorative Dentistry & Endodontics
Year:
2016
Type:
Article
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