The effects of integrin β1 antisense oligodeoxynucleotide on the expression of F-actin in ECV-304 cells exposed to infrasound / 中华物理医学与康复杂志

ABSTRACT

Objective To study the effect of integrin β1 antisense oligodeoxynucleotide(ASODN)on the expression of F-actin in ECV-304 ceils exposed to infrasound,and to explore the pathway of infrasound signal transfer into the cells.Methods integrin β1 oligodeoxynucleotides were embedded in cationic liposome lipofectamine 2000 reagent and transfected into ECV-304 cells.Laser scanning confoeal microscopv was used to observe F-actin's expression when the cells were exposed to 16 Hz infrasound at 130 dB for 2 h.The cells' average fluorescence was examined using spectrofluorimetric quantification after immunofluorescent staining. Results Among the three false exposure groups,most fluorescein-labelled substances in the cells were in a diffuse state.There were few actin filaments,and they were tenuous and short, without direction.The cells' fluorescence intensities were almost the same.In a group exposed to infrasound,F-actin was thick and long with a longitudinal arrangement.Fluorescence intensities were strong.However,less F-actin expression was observed in the ceils of the ASODN group exposed to infrasound compared with the others similarly exposed.Correspondingly.F-actin expression in the ASODN group exposed to infrasound was almost the same as in the other exposed group.Conclusion F-actin expression can be increased in ECV-304 cells by infrasound exposure,but integrin β1 ASODN may partially inhibit its expression.The extraeellular matrix-integrin-cytoskeleton may be one of the transduetion pathways for infrasound signals into the cells.