Ozon-induced airway hyperresponsiveness of rat tracheal smooth muscle / 천식및알레르기
Journal of Asthma, Allergy and Clinical Immunology
;
: 19-30, 1999.
Article
in Korean
| WPRIM
| ID: wpr-38135
ABSTRACT
BACKGROUND:
Ozone (03) induces airway inflammation and hyperresponsiveness which are characteristic features of asthma. There have been few studies observing O3-induced increase in responsiveness of rat airway muscle.Objectives:
The aims of this study were to develop an O3-induced nonallergic asthma model using rat tracheal smooth muscle (TSM) and to evaluate the role of airway epithelium on the modulation of muscle responsiveness.METHOD:
Five groups of 20 male Sprague-Dawley(SD) rats were exposed to filtered air including 0.12, 0.5, 1.0, or 2.0 ppm 03 for 1 hour. Thirty minutes after the exposure, bronchoalveolar lavage (BAL) and isometric contractile responses of the isolated tracheal ring segments to KCI, acetylcholine (ACh), and electrical field stimulation (EFS) were measured.RESULTS:
The percent age of neutrophils was significantly higher and that of alveolar macro-phages in BAL fluid was significantly lower in 2.0 ppm O3-exposed rats than in the control. There were no significant differences in the maximal contractile responses of TSM to KC1, ACh, EFS and in the sensitivity to ACh (ACh-EC50) and EFS (EFS-EC50) between the control group and the ozone exposed group. ACh-EC50 and EFS-EC50 were correlated positively with the percent age of neutrophils and inversely with that of macrophages. Removal of epithelium significantly increased the sensitivity to ACh in O3-exposed group, but not in the control group.CONCLUSION:
These findings indicate that O3 induces neutrophilic airway inflammation, but not an increased sensitivity of TSM to ACh or EFS in SD rats. However, O3-induced epithelial damage may be associated with increased muscle response.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ozone
/
Asthma
/
Trachea
/
Acetylcholine
/
Bronchoalveolar Lavage
/
Epithelium
/
Inflammation
/
Macrophages
/
Muscle, Smooth
/
Neutrophils
Type of study:
Prognostic study
Limits:
Animals
/
Humans
/
Male
Language:
Korean
Journal:
Journal of Asthma, Allergy and Clinical Immunology
Year:
1999
Type:
Article
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