Transfer of regulatory T cells induced by TGF-β1prolonged the skin-graft survival in mice / 中华微生物学和免疫学杂志

ABSTRACT

Objective To study the probability of transferring the regulatory T cells induced by TGF-β1 to prolong the allograft survival and the mechanisms involved.Methods According to the different culture conditions.three experimental groups were established:control group(T cells from C57 BL/6 mice cultured with II-2),MLR group(T cells from C57BL/6 mice activated by alloantigen)and TGF-βgroup(T cells from C57BL/6 mice activated by alloantigen and cultured with 5.0 ng/ml TGF-β1).After the culture,the ratio of CI4+CD25+T and the Foxq3 expression were measured by FACS and RT-PCR,respectively.On 9th day,the pathologic analysis was performed and the ratios of TH1,TH2 and Treg and the proliferation of lymphocytes were measured.Results The ratio of CD4+CD25+T in TGF-β group was higher than that in control group and MLR group(P<0.05),and Foxp3 was expressed in CD4+CD25+T cell from TGF-βgroup.After transferring ofthe cells,the allografi survival time in TGF-β group was prolonged and its mean survival time(MST)was(22.8±1.9)d,which was longer than that in MLR group and control group (P<O.05),but MST of MLR group was(9.4 ±1.3)d,which was shorter than that of control group(P<0.05).In TGF-β group,the ratio of THl was lower than that of control group and MLR group(P<O.05), but the ratio of TH2 was similar with MLR group(P>0.05),lower than that of control group(P<0.05). And the ratio of CD4+CD25+T in TGF-β group was higher than that in control and MLR group obviously(P<O.05).In addition,the proliferation of lymphocytes in TGF-β group was impaired.Conclusion TGF-β1 could induce na(i)ve T cens to difierentiate into regulatory T cells.After transferring these cells in mice skin transplant model,the ratio of CD4+CD25+Treg waS increased,and the differentiation of TH1 and TH2 and the proliferation of lymphocytes were inhibited.Finally.the allograft survival time was prolonged successfully.