Apoptotic mechanism of WJH-6, a novel polyamine conjugate, on K562 and HL-60 cells / 药学学报
Acta Pharmaceutica Sinica
; (12): 451-5, 2010.
Article
in Zh
| WPRIM
| ID: wpr-382329
Responsible library:
WPRO
ABSTRACT
In the present study, the apoptotic mechanism and polyamine transporter recognition of WJH-6, a novel polyamine conjugate, were investigated in K562 and HL-60 cells. The cytotoxicity of WJH-6 was assessed by MTT assay; cell cycle distribution and apoptosis were measured by flow cytometry; the protein expression of Caspase-3, Caspase-8, Caspase-9, Bid and mitochondrial membrane potential (MMP) were evaluated by high content screening (HCS) analysis; the protein expression of cytochrome c was measured by Western blotting. The results showed that WJH-6 could be recognized and transported by polyamine transporter (PAT). Furthermore, WJH-6 was able to inhibit K562 and HL-60 cells proliferation and induce apoptosis. This apoptotic effect was relative to MMP loss, cytochrome c release from mitochondria to cytoplasm and the activation of Caspase-8, Caspase-9, Caspase-3 and Bid. These results suggested that WJH-6-induced K562 and HL-60 cells apoptosis was related with mitochondrial damage.
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WPRIM
Language:
Zh
Journal:
Acta Pharmaceutica Sinica
Year:
2010
Type:
Article