Effects of cholinergic receptor antagonists, adrenergic drugs and pethidine on the function of sphincter of Oddi isolated from rabbits / 中华消化杂志

ABSTRACT

Objective To investigate the contractive effect of atropine, noradrenaline,adrenaline and pethidine on sphincter of Oddi (SO) isolated from rabbits. Methods The rings of SO isolated from 60 rabbits were treated with Krebs solution and then were exposed to gradient atropine,anisodamin, noradrenalin, adrenaline or pethidine with 10 each. The rest 10 rings of SO treated with Krebs solution only were served as controls. The amplitude and frequency of contraction of SO were recorded. Results Compared with control group, all of the 5 medicines were able to significantly decrease the contractive amplitude, but not frequency, of SO at the concentrations ranged from 10-6 mol/L to 10-2 mol/L (P＜0.05). The inhibition order was as follows noradrenaline ＞ adrenaline ＞atropine ＞ anisodamin ≈ pethidine. Conclusions Beside atropine and anisodamin, noradrenaline,adrenaline and pethidine also showed the direct relaxation of SO by decreasing contractive amplitude of SO. Adrenaline or noradrenaline displayed stronger effect in decreasing contractive amplitude of SO when compared with atropine or anisodamin.