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Effects of Different Scaffolds and Cultures on Growth of Preadipocytes / 中华医学美学美容杂志
Article in Zh | WPRIM | ID: wpr-383426
Responsible library: WPRO
ABSTRACT
Objective To culture preadipocytes in vitro and to study the cell compatibility of PLGA scaffolds,collagen scarfolds and hyaluronic acid-based scaffolds and tO choose the optimal seeding method.Methods The preadipocytes from human abdominal adipose tissue were isolated and cultured in enzyme-digesting method.The generation of human preadipocytes was planted on PLGA scaffolds,collagen scaffolds and hyaluronic acid-based scaffolds.and the cell compatibility was observed by MTT method.The seeding efficiency of human preadipocytes on scaffolds.human preadipocytes were seeded to hyaluronic acid-based scaffolds by static culture and stirred culture.Results Compared compatibility of preadipocyte with three different scaffolds,there was great difference between hyaluronic acid-based scaffolds and PLGA scaffolds.Difference also existed between hyaluronic acid-based scaffolds and collagen scaffolds that were different from PLGA scaffolds.Among them,hyaluronic acid-based scaffolds was the best.Conclusion Hyaluronic acid iS a better scaffolds material for adipose tissue engineering compared with PLGA and collagen.The seeding efficiency of stirred culture is higher than static culture,which is an optimal method for cell seeding tO 3-D scaffolds.
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Full text: 1 Index: WPRIM Language: Zh Journal: Chinese Journal of Medical Aesthetics and Cosmetology Year: 2010 Type: Article
Full text: 1 Index: WPRIM Language: Zh Journal: Chinese Journal of Medical Aesthetics and Cosmetology Year: 2010 Type: Article