Effect of DCN gene on the anti-tumor and immunological functions of mice bearing S180 sarcoma / 肿瘤研究与临床

ABSTRACT

Objective To investigate the anti-tumorigenesis function of DCN gene and its effect on the immunological funotions of mice bearing S180.Methods Twenty four mice bearing S180 were divided into three groups at random(eight mice every group),and then the normal saline group(negative control),pcDNA3.1(+)vector group and pcDNA3.1(+)-DCN group were set up for the experiment.They all started to inject next day by armpit.The volume of tumors were measured at the 5th day,7th day,9th day,11th day.The transformation rate of T lymphocytes were detected by means of MTT,the mRNA level of TGF-β by RT-PCR and the level of VEGF in serum by ELISA.Results The average volume of tumor in pcDNA3.1 (+)-DCN group were deflated to(2.219±0.105)cm3 comparing with the others(P＜0.001);the transformation rate of T lymphocytes were increased significantly to(67.21±6.32)%(P＜0.01,P＜0.05);meanwhile it can decrease the mRNA level of TGF-β in serum,and depress the expression of VEGF markedly,whose absorbance were2.6175±0.099(P＜0,01,P＜0.05).Conclusion DCN gene could significantly inhibit the growth of tumor,and enhance the immunological function of mice bearing S180 in vivo.