Expression of various subtypes of HLA-G mRNA in placentas of pregnant women complicated with severe preeclampsia / 中华围产医学杂志

ABSTRACT

Objective To investigate the different expressions of various subtypes of human leukocyte antigen-G(HLA-G)mRNA in placentas among early and late-onset severe preeclamptic and normal pregnant women. Methods Eleven placental samples from early-onset severe preeclamptic pregnancies and fourteen from late-onset severe preeclamptic pregnancies were collected as study group;eight placental samples from normal pregnancies were collected as control group.The levels of HLA-G mRNA in the three groups were measured by nested RT-PCR. Results Compared with the counterparts in the control group,the leveI of HLA-G1 subtype was significantly decreased (medianEarly-onset group1.37,P<0.05;Late-onset group24.90,P<0.05;Control group46.67,P<0.05)and the level of HLA-G5 subtype increased(medianEarly-onset group 19.23;Late-onset group3.65;Control group1.33)in both early and late-onset severe preeclamptic group.The level of HLA-G2 subtype increased(medianEarly-onset group0;Late-onset group21.59,P<0.05;Control group5.39)in the late-onset severe preeclamptic group. Conclusion The reduced expression of HLA-G membrane-bound subtype mRNA in the placenta and the increased expression of HLA-G soluble subtype mRNA in the placenta may be related tO the pathogenesis of preeclampsia.