Inducible IL-12 gene expression mediated by naked DNA containing an RU486 regulatory system / 中华微生物学和免疫学杂志
Chinese Journal of Microbiology and Immunology
;
(12): 102-106, 2008.
Article
in Chinese
| WPRIM
| ID: wpr-384111
ABSTRACT
Objective To evaluate the inducibility of target gene expression induced by plasmid DNA carrying a RU486 regulatory system. Methods Plasmid pRS-LacZ encoding LacZ gene and plasmid pRS22 encoding murine interleukin-12(IL-12)gene were used to bansfect cells in vitro or be rapidly injected into the vein of mice. Then LacZ expression was assayed in cultured cells and tissues by X-gal staining.The IL-12 level was tested in the supernatants of cultured cells and in the serum with ELISA. ResultsIn the presence of RU486.30% of the liver derived hepatic HepG2 cells were dyed blue while less than 1%of the HepG2 cells were blue colored in the absence of RU486. Less than 0.5% of other non-hepatic cells were in blue with or without RU486. After transfection, the IL-12 level in the supernatants of HepG2 cells increased with increasing concentrations of RU486 treatment. After intravenous injection of plasmid pRS-LacZ,blue colored cells were observed only in liver tissue while induced by RU486. After intravenous injection of plasmid pRS22,the IL-12 expression level in serum was related with the dosage of RU486 or/and plasmid DNA. Conclusion Plasmid DNA containing RU486 regulatory system could open or close IL-12 gene expression by the addition or deletion of RU486. And the IL-12 expression level in serum could be regulated by altering the dose of plasmid DNA or/and RU486.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Microbiology and Immunology
Year:
2008
Type:
Article
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