The study of the correlation between the IFN-γ mRNA expression in heart allograft and postoperative time / 中华胸心血管外科杂志

ABSTRACT

Objective: This study was designed to determine the expression of IFN-γ mRNA in heart allograft and its correlation to postoperative, cell infiltrating and allograft survival time. Methods: The heterotopic rat heart transplantation model was established with a simplified method, and the allografts and isografts were harvested at 1, 3,5,7,9 and 11 postoperative days. After the total RNA was prepared, the standard primer and target primer were amplified in the same tube, the average ratio of the target products pixel volume to primer's was calculated and a coordinates chart was made. Results: The expression level of IFN-γ mRNA in heart allograft was elevated and its peak was on the fifth postoperative day, and prior to the heart death has a positive correlation with cell infiltrating. Conclusion: IFN-γ may play on important role in postoperative rejection.