Effects of Netrin-1 on angiogenesis in vitro and in vivo / 中国综合临床
Clinical Medicine of China
;
(12): 1265-1267, 2010.
Article
in Chinese
| WPRIM
| ID: wpr-385193
ABSTRACT
Objective To investigate the effects of Netrin-1 on angiogenesis in vitro and in vivo. Methods We performed in vitro rat aortic ring assay and in vivo Matrigel plug assay to determine the effect of Netrin-1 on angiogenesis. Results 10 μg/L, 50 μg/L and 100 μg/L Netrin-1 stimulated microvessel sprouting from the adventitia of aortic rings and the effect reached maximum at 50 μg/L The in vivo Matrigel plug assay showed orange color change if Nestrin-1 was positive; and CD34 immunofluorescent staining showed vascular structures in the Matrigel plug with hemachrome ( 53.4 ± 7. 3 ), which was significantly higher than control ( 5. 8 ± 0. 9 )Conclusions Netrin-1 can induce angiogenesis in vitro and in vivo.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Clinical Medicine of China
Year:
2010
Type:
Article
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