Impact of hirudin on the numbers of CD34 positive microvessel and RCA-1 positive cell and neutrophil in perihematoma tissue after intracerebral hemorrhage in rats / 国际脑血管病杂志

ABSTRACT

Objective To observe the dynamic changes of the numbers of CD34 positive microvessel and RCA-1 positive cell and neutrophil in perihematoma tissue after intracerebral hemorrhage (ICH) in rats at different time points and the impact of thrombin-specific inhibitor hirudin on the above different indicators and to investigate the protective mechanisms of hirudin for brain injury following ICH. Methods An experimental model was made with autologous whole blood injecting into the rat brain basal ganglia by using the stereotactic method. The rats were randomly assigned to sham operation, ICH, hirudin intervention, and normal saline groups.CD34 and RCA-1 immunobistochemistry stainings and conventional HE staining were used to observe CD34-positive microvessel, RCA-1 positive cell and neutrophil.Results The numbers of CD34-positive microvessel began to decrease at 12 hours after ICH, it decreased to the lowest at 72 hours, and it gradually returned to normal levels at day 7. The RCA-1 positive cells could be observed at 6 hours after ICH. It reached the peak at 48 hours. A small amount could persist for two weeks. Neutrophil could be observed at 12 hours after ICH. It reached the peak at 48 hours and disappeared at week 2. The administration of hirudin significantly reduced the numbers of RCA-1 positive cell and neutrophil in the early stage of ICH (5 min). At the same time, it significantly inhibited the decreased numbers of CD34-positive microvessel (all P ＜0. 01). The administration of hirudin during the edema formation also significantly reduced the numbers of RCA-1 positive cell and neutrophil (all P＜ 0.05), however, it could not significantly increase the numbers of CD34-positive microvessel. Conclusions Thrombinmediated inflammatory response has involved in the process of brain injury after ICH, and early administration of hirudin may significantly relieve perihematoma tissue injury.