Analysis of HPV-other Samples by Performing HPV DNA Sequencing
Korean Journal of Pathology
;
: 250-253, 2009.
Article
in English
| WPRIM
| ID: wpr-38537
ABSTRACT
BACKGROUND:
HPV-other samples are designated as being positive on HPV-PCR, but negative when using specific HPV hybridization probes. We wanted to determine the types on the HPV-other samples by performing sequencing, and to know the pathologic status of the uterine cervix according to the HPV type detected on sequencing.METHODS:
For HPV genotying, we used the commercially available HPV DNA Chip test, which contains 15 types of high-risk HPV and 9 types of low-risk HPV. The HPV DNA sequencing was performed for the HPV-other samples of 209 patients who subsequently underwent cervical biopsy.RESULTS:
For 204 of the 209 samples, the HPV types detected by sequencing were absent types at used HPV DNA chip. For the remaining 5 samples, sequencing was impossible due to mixed peaks. HPV-81 (19.6%), HPV-61 (18.6%), HPV-62 (16.7%) and HPV-84 (13.9%) were frequently detected. For the HPV-81, -62, -71, and -72 samples, most of the samples displayed normal or LSIL. However, HPV-84 and -61 were more associated with HSIL or worse, as compared to the other types.Conclusion:
HPV-81, -61, -62 and -84 were frequently found on sequencing analysis of the HPV-other samples. The pathologic status was diverse, according to the HPV type detected on sequencing.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Papillomaviridae
/
Biopsy
/
DNA
/
Cervix Uteri
/
Chimera
/
Sequence Analysis, DNA
/
Oligonucleotide Array Sequence Analysis
Limits:
Female
/
Humans
Language:
English
Journal:
Korean Journal of Pathology
Year:
2009
Type:
Article
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