Effect of propofol pretreatment on hypoxia-induced apoptosis of alveolar epithelial type Ⅱ cells in fetal rats / 中华麻醉学杂志
Chinese Journal of Anesthesiology
; (12): 865-867, 2010.
Article
in Zh
| WPRIM
| ID: wpr-386071
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WPRO
ABSTRACT
Objective To investigate the effect of propofol pretreatment on hypoxia-induced apoptosis of alveolar epithelial type Ⅱ (AE Ⅱ) cells in fetal rats. Methods Primary cultured AE Ⅱ cells isolated from fetal rats were seeded in 96-well plates (1 × 106/L, 180 μl/well) and randomly assigned to one of 3 groups (n = 72each):normal control group (group C), hypoxia group (group H) and propofol-hypoxia group (group P-H).Group H and P-H were exposed to hypoxia (5% O2). In group P-H, propofol (final concentration 5 μ mol/L) was added 1 h prior to hypoxia (5% O2). The apoptotic rate and expression of hypoxia-inducible factor (HIF)-1αmRNA, Bnip3L mRNA, HIF-1α protein and Bnip3L protein were determined at 3, 12, 24 and 48 h of hypoxia.Results The apoptotic rate and expression of HIF-1α mRNA, Bnip3L mRNA, HIF-lα protein and Bnip3L protein were significantly up-regulated in group H compared with group C (P < 0.05). Propofol pretreatment could significantly inhibit the hypoxia-induced changes mentioned above (P < 0.05). Conclusion Propofol pretreatment can inhibit hypoxia-induced apoptosis of AE Ⅱ cells, and the mechanism is related to inhibition of HIF-1αactivation and down-regulation of Bnip3L expression in fetal rats.
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WPRIM
Language:
Zh
Journal:
Chinese Journal of Anesthesiology
Year:
2010
Type:
Article