Role of stromal cell-derived factor-1α in inducing recruitment of bone marrow-derived cells to cutaneous wound area / 中华创伤杂志

ABSTRACT

Objective To explore the effect of stromal cell-derived factor-1 α (SDF-1 α) on inducing recruitment of bone marrow-derived cells (BMDCs) to wound area. Methods BMDCs were isolated from bone marrow, cultured with routine method and identified by CXCR4 antibody. Cells cultured with CXCR4 antibody (100 ng,/mL) for 6 hours were labeled with CM-DiI and injected into the tail vein of full-thickness incisional wound model (set as anti-CXCR4 group). BMDCs labeled with CM-DiI without antibody treatment were injected to the rats in BMDCs group, and rats were injected with DMEM/F12 serum-free medium in the control group. The quantity of labeled BMDCs at the wound site and the percentage of wound closure were measured. Results (1) All BMDCs expressed CXCR4. (2) The percentages of wound closure at days 7 and 14 in BMDCs group (7 d 41.3% ±4.6%; 14 d92.3% ±2. 1%) were significantly higher than those of control group (7 d 29.3% ±2. 3%; 14 d 77.3% ±2.5%) and anti-CXCR4 group (7 d 30.7% ±4.6% ;14 d 85.7% ±1.5%) (P＜0.05). The percentage of wound closure of anti-CXCR4 group was significantly higher than that of control group at day 14(P ＜ 0.05). (3) The number of CM-DiI labeled BMDCs at wound site at days 7 and 14 in BMDCs group [7 d (535 ±84) cells/hpf; 14 d (769 ±124) cells/hpf) were greater than those of anti-CXCR4 group [7 d (335 ±97) cells/hpf; 14 d (521 ± 127) cells/hpf] (P＜0.05). Conclusions BMDCs participate in the cutaneous wound healing. SDF-1α plays an important role in recruiting BMDCs to wound area.